|DNA is depicted in blue and Bgl II is orange|
|DNA is Blue and each unit it a different shade of orange|
|From this angle, the loops depicted in dark orange are visibly interacting|
Bgl II scans the DNA from the major groove side and approaches the DNA from the major groove side. The enzyme uses a scissor like motion to bind with the DNA unlike other type two endonuclease restriction enzymes in its classification. When it is in the relaxed free position, the enzyme looks like this:
When Bgl II is bound to DNA it is in this position:
In addition to the scissor like motion, the loops that enclose the DNA are bent back in the free position, then bend over the DNA when Bgl II is interacting with the DNA.
Ions present in solution with Bgl II are important to the activity. The ion Mg 2+ activates Bgl II the most, and the presence of NaCl aids in the activity.
Bgl II binds and cleaves the same section of DNA as Bgl I. Type two restriction endonucleases cleave DNA without the use of ATP. Bgl II specifically cleaves a section leaving sticky ends. The place where the DNA is broken is marked with orange arrows in the picture below:
Bgl II does not significantly unwind the DNA when they are interacting and cleaves both Strands of DNA simultaneously, which is not common for restriction endonucleases.
Bgl II is used in various experiments. One use is to break down large segments of DNA to be analyzed with Gel Electrophoresis. Also with this use, it is possible to determine if the site exists that Bgl II cleaves by seeing if the DNA traveled on the Gel; if there are multiple places where Bgl II cleaves, then there would be multiple small fragments. Endonuclease restriction enzymes are commonly used in Biotechnology experiments. Specifically, Bgl II is used in the insertion of gene segments into a section of DNA, such as a plasmid, or the deletion of a segment. This use of Bgl II is important in the replication of genes and the study of the results of changing DNA sequences.
The funny thing when talking about this protein is what do you call it: Bgl II
|Photos taken from various blog posts then edited for resulting pictures|
(All images were created for this page. The last image was two pictures taken from various blogs, then edited for this blog)
- Lukacs, Christine M., Rebecca Kucera, Ira Schildkraut, and
Aneel k. Aggarwal. "Structure of Free BglII reveals an unpredicted
scissor-like motion for opening and endonuclease." Nature Publishing
Group 8.2 (2001). Print.
Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine
- Imber, Roland, and Thomas A. Bickle. "Purification and
Properties of the Restriction Endonuclease BglII from Baccillus globigii."
Federation of the Societies of Biochemistry and Molecular Biology 117
(1981): 395-99. Print.
Microbiology Department, Biozentrum, University of Basel
Pingoud, Alfred, and Albert Jeltsch. "Structure and Function of Type 2 Restricstion Endonucleases." Nucleic Acids Reasearch 29.18 (2001): 3705-27. Print.Institut für Biochemie (FB 08), Justus-Leibig-Univerität, Heinrich-Buff-Ring 58, D-35392 Giessen, Germany